Fast genome editing in Bacillus subtilis
نویسندگان
چکیده
منابع مشابه
Bacillus subtilis genome editing using ssDNA with short homology regions
In this study, we developed a simple and efficient Bacillus subtilis genome editing method in which targeted gene(s) could be inactivated by single-stranded PCR product(s) flanked by short homology regions and in-frame deletion could be achieved by incubating the transformants at 42°C. In this process, homologous recombination (HR) was promoted by the lambda beta protein synthesized under the c...
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ژورنال
عنوان ژورنال: Engineering in Life Sciences
سال: 2019
ISSN: 1618-0240,1618-2863
DOI: 10.1002/elsc.201800164